5HR6

X-ray crystal structure of C118A RlmN with cross-linked tRNA purified from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	RlmN methylase	polymer	404	45296.8	2	UniProt (A7ZPW0) Pfam (PF21016) Pfam (PF04055) UniProt (by SIFTS) (P36979)	Escherichia coli	23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase
2	B, D (C, D)	tRNA Glu	polymer	68	21808.0	2	GenBank (937521852)	Escherichia coli
3	E, L (A, B)	METHIONINE	non-polymer		149.2	2	Chemie (MET)
4	F, M (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
5	G, N (A, B)	5'-DEOXYADENOSINE	non-polymer		251.2	2	Chemie (5AD)
6	H, I, J, K, O... (A, C, B, D)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	Q, R, S, T (A, C, B, D)	water	water		18.0	46	Chemie (HOH)

Sequence modifications

A, B: 1 - 384 (UniProt: A7ZPW0)

PDB	External Database	Details
Ala 118	Cys 118	engineered mutation
Gly 385	-	expression tag
Asn 386	-	expression tag
Ser 387	-	expression tag
Ser 388	-	expression tag
Ser 389	-	expression tag
Val 390	-	expression tag
Asp 391	-	expression tag
Lys 392	-	expression tag
Leu 393	-	expression tag
Ala 394	-	expression tag
Ala 395	-	expression tag
Ala 396	-	expression tag
Leu 397	-	expression tag
Glu 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134209.5
Non-Polymers*	Number of molecules	12
	Total formula weight	1650.0
All*	Total formula weight	135859.5

*Water molecules are not included.