5HR6
X-ray crystal structure of C118A RlmN with cross-linked tRNA purified from Escherichia coli
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | RlmN methylase | polymer | 404 | 45296.8 | 2 | UniProt (A7ZPW0) Pfam (PF21016) Pfam (PF04055) UniProt (by SIFTS) (P36979) In PDB | Escherichia coli | 23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase |
2 | C, D | tRNA Glu | polymer | 68 | 21808.0 | 2 | GenBank (937521852) | Escherichia coli | |
3 | A, B | METHIONINE | non-polymer | 149.2 | 2 | Chemie (MET) | |||
4 | A, B | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
5 | A, B | 5'-DEOXYADENOSINE | non-polymer | 251.2 | 2 | Chemie (5AD) | |||
6 | A, C, B, D | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
7 | water | water | 18.0 | 46 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 384 (UniProt: A7ZPW0)
PDB | External Database | Details |
---|---|---|
Ala 118 | Cys 118 | engineered mutation |
Gly 385 | - | expression tag |
Asn 386 | - | expression tag |
Ser 387 | - | expression tag |
Ser 388 | - | expression tag |
Ser 389 | - | expression tag |
Val 390 | - | expression tag |
Asp 391 | - | expression tag |
Lys 392 | - | expression tag |
Leu 393 | - | expression tag |
Ala 394 | - | expression tag |
Ala 395 | - | expression tag |
Ala 396 | - | expression tag |
Leu 397 | - | expression tag |
Glu 398 | - | expression tag |
His 399 | - | expression tag |
His 400 | - | expression tag |
His 401 | - | expression tag |
His 402 | - | expression tag |
His 403 | - | expression tag |
His 404 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 134209.5 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 1650.0 | |
All* | Total formula weight | 135859.5 |