5HR6
X-ray crystal structure of C118A RlmN with cross-linked tRNA purified from Escherichia coli
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, B) | RlmN methylase | polymer | 404 | 45296.8 | 2 | UniProt (A7ZPW0) Pfam (PF21016) Pfam (PF04055) UniProt (by SIFTS) (P36979) | Escherichia coli | 23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase |
| 2 | B, D (C, D) | tRNA Glu | polymer | 68 | 21808.0 | 2 | GenBank (937521852) | Escherichia coli | |
| 3 | E, L (A, B) | METHIONINE | non-polymer | 149.2 | 2 | Chemie (MET) PubChem (876) PubChem (6137) PubChem (84815) | |||
| 4 | F, M (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 5 | G, N (A, B) | 5'-DEOXYADENOSINE | non-polymer | 251.2 | 2 | Chemie (5AD) PubChem (142) PubChem (71678) PubChem (439182) PubChem (449044) PubChem (1277776) PubChem (6398669) PubChem (6928544) PubChem (12827542) PubChem (16725083) PubChem (23638944) PubChem (25201172) PubChem (25796474) PubChem (25796475) PubChem (40467848) PubChem (23615209) PubChem (40467849) PubChem (676784) PubChem (6541038) PubChem (57115448) PubChem (59731278) PubChem (90321697) PubChem (121275112) PubChem (124363198) PubChem (133636852) PubChem (140689696) PubChem (142801875) PubChem (142801934) | |||
| 6 | H, I, J, K, O... (A, C, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 7 | Q, R, S, T (A, C, B, D) | water | water | 18.0 | 46 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 384 (UniProt: A7ZPW0)
| PDB | External Database | Details |
|---|---|---|
| Ala 118 | Cys 118 | engineered mutation |
| Gly 385 | - | expression tag |
| Asn 386 | - | expression tag |
| Ser 387 | - | expression tag |
| Ser 388 | - | expression tag |
| Ser 389 | - | expression tag |
| Val 390 | - | expression tag |
| Asp 391 | - | expression tag |
| Lys 392 | - | expression tag |
| Leu 393 | - | expression tag |
| Ala 394 | - | expression tag |
| Ala 395 | - | expression tag |
| Ala 396 | - | expression tag |
| Leu 397 | - | expression tag |
| Glu 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
| His 402 | - | expression tag |
| His 403 | - | expression tag |
| His 404 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 134209.5 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1650.0 | |
| All* | Total formula weight | 135859.5 |
