5HR6
X-ray crystal structure of C118A RlmN with cross-linked tRNA purified from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.664, 69.821, 149.298 |
Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
Resolution | 88.663 - 2.880 |
R-factor | 0.18365 |
Rwork | 0.182 |
R-free | 0.22811 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.695 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.663 | 88.663 | 2.900 |
High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
Rmerge | 0.132 | 0.066 | |
Number of reflections | 23364 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 99.6 | 99.2 | 99.6 |
Redundancy | 6.3 | 5.1 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, potassium chloride, magnesium chloride, Tris-HCl |