5HQN

Catalytic domain of murine Acid Sphingomyelinase (ASMase, ASM, SMPD1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Sphingomyelin phosphodiesterase	polymer	473	53640.6	2	UniProt (Q04519) Pfam (PF00149) Pfam (PF19272)	Mus musculus (Mouse)	Acid sphingomyelinase,aSMase
2	C, E, H (C, E, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	D, F, G (D, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	3	In PDB GlyTouCan (G21290RB)
4	I, J, L, M (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, O (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6	N (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7	P, Q (A, B)	water	water		18.0	74	Chemie (HOH)

Sequence modifications

A, B: 165 - 627 (UniProt: Q04519)

PDB	External Database	Details
Asp 155	-	expression tag
Arg 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
Lys 163	-	expression tag
Leu 164	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	107281.2
Branched	Number of molecules	6
	Total formula weight	2984.8
Non-Polymers*	Number of molecules	7
	Total formula weight	672.8
All*	Total formula weight	110938.8

*Water molecules are not included.