5HPY
Crystal Structure of RhoA.GDP.MgF3-in complex with human Myosin 9b RhoGAP domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, D) | Unconventional myosin-IXb | polymer | 232 | 26060.3 | 2 | UniProt (Q13459) Pfam (PF00620) | Homo sapiens (Human) | Unconventional myosin-9b |
| 2 | B, D (B, F) | Transforming protein RhoA | polymer | 185 | 20806.7 | 2 | UniProt (P61586) Pfam (PF00071) | Homo sapiens (Human) | Rho cDNA clone 12,h12 |
| 3 | E, H (B, F) | TRIFLUOROMAGNESATE | non-polymer | 81.3 | 2 | Chemie (MGF) | |||
| 4 | F, J, K (B, F) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | G, I (B, F) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 6 | L, M, N, O (A, B, D, F) | water | water | 18.0 | 136 | Chemie (HOH) |
Sequence modifications
A, D: 191 - 416 (UniProt: Q13459)
B, F: 3 - 181 (UniProt: P61586)
| PDB | External Database | Details |
|---|---|---|
| Gly 185 | - | expression tag |
| Pro 186 | - | expression tag |
| Gly 187 | - | expression tag |
| Ser 188 | - | expression tag |
| Glu 189 | - | expression tag |
| Phe 190 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Glu 1 | - | expression tag |
| Phe 2 | - | expression tag |
| Asn 25 | Phe 25 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 93733.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1121.9 | |
| All* | Total formula weight | 94855.8 |
