5HPY

Crystal Structure of RhoA.GDP.MgF3-in complex with human Myosin 9b RhoGAP domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Unconventional myosin-IXb	polymer	232	26060.3	2	UniProt (Q13459) Pfam (PF00620) In PDB	Homo sapiens (Human)	Unconventional myosin-9b
2	B, F	Transforming protein RhoA	polymer	185	20806.7	2	UniProt (P61586) Pfam (PF00071) In PDB	Homo sapiens (Human)	Rho cDNA clone 12,h12
3	B, F	TRIFLUOROMAGNESATE	non-polymer		81.3	2	Chemie (MGF)
4	B, F	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	B, F	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
6		water	water		18.0	136	Chemie (HOH)

Sequence modifications

A, D: 191 - 416 (UniProt: Q13459)

PDB	External Database	Details
Gly 185	-	expression tag
Pro 186	-	expression tag
Gly 187	-	expression tag
Ser 188	-	expression tag
Glu 189	-	expression tag
Phe 190	-	expression tag

B, F: 3 - 181 (UniProt: P61586)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Glu 1	-	expression tag
Phe 2	-	expression tag
Asn 25	Phe 25	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	93733.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1121.9
All*	Total formula weight	94855.8

*Water molecules are not included.