5HPY
Crystal Structure of RhoA.GDP.MgF3-in complex with human Myosin 9b RhoGAP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.055, 90.762, 93.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.698 - 2.400 |
R-factor | 0.2126 |
Rwork | 0.211 |
R-free | 0.24620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ow3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.257 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 38652 |
<I/σ(I)> | 12.8 |
Completeness [%] | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% (w/v) PEG 3350, 0.2M sodium malonate pH 7.0. |