5HIA

Human hypoxanthine-guanine phosphoribosyltransferase in complex with [3R,4R]-4-guanin-9-yl-3-((S)-2-hydroxy-2-phosphonoethyl)oxy-1-N-(phosphonopropionyl)pyrrolidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Hypoxanthine-guanine phosphoribosyltransferase	polymer	224	25377.2	4	UniProt (P00492) Pfam (PF00156) In PDB	Homo sapiens (Human)	HGPRTase
2	A, B, C, D	[3-[(3~{R},4~{R})-3-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[(2~{S})-2-oxidanyl-2-phosphono-ethoxy]pyrrolidin-1-y l]-3-oxidanylidene-propyl]phosphonic acid	non-polymer		496.3	4	Chemie (YPG)
3	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4		water	water		18.0	444	Chemie (HOH)

Sequence modifications

A, B, C, D: 0 - 217 (UniProt: P00492)

PDB	External Database	Details
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ala 22	Cys 23	engineered mutation
Ala 205	Cys 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101508.7
Non-Polymers*	Number of molecules	12
	Total formula weight	2179.7
All*	Total formula weight	103688.3

*Water molecules are not included.