5HHT

Crystal structure of E. coli transketolase triple variant Ser385Tyr/Asp469Thr/Arg520Gln

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transketolase 1	polymer	669	73154.5	2	UniProt (P27302) Pfam (PF00456) Pfam (PF02779) Pfam (PF02780) In PDB	Escherichia coli K-12	TK 1
2	B, A	1,2-ETHANEDIOL	non-polymer		62.1	36	Chemie (EDO)
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	B, A	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
5		water	water		18.0	1750	Chemie (HOH)

Sequence modifications

A, B: 1 - 663 (UniProt: P27302)

PDB	External Database	Details
Tyr 385	Ser 385	engineered mutation
Thr 469	Asp 469	engineered mutation
Gln 520	Arg 520	engineered mutation
His 664	-	expression tag
His 665	-	expression tag
His 666	-	expression tag
His 667	-	expression tag
His 668	-	expression tag
His 669	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	146309.1
Non-Polymers*	Number of molecules	40
	Total formula weight	3165.2
All*	Total formula weight	149474.3

*Water molecules are not included.