5HBB

Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG - E139A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (B, A, C)	Cell surface protein SpaA	polymer	283	30665.5	3	UniProt (C7T9P4)	Lactobacillus rhamnosus GG
2	D, L, T (B, A, C)	THIOCYANATE ION	non-polymer		58.1	3	Chemie (SCN)
3	E, F, G, H, I... (B, A, C)	SODIUM ION	non-polymer		23.0	17	Chemie (NA)
4	J, S, Z (B, A, C)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	AA, BA, CA (B, A, C)	water	water		18.0	286	Chemie (HOH)

Sequence modifications

B, A, C: 35 - 302 (UniProt: C7T9P4)

PDB	External Database	Details
Met 28	-	expression tag
Gly 29	-	expression tag
Arg 30	-	expression tag
Asp 31	-	expression tag
Pro 32	-	expression tag
Asn 33	-	expression tag
Ser 34	-	expression tag
Ala 139	Glu 139	engineered mutation
Leu 303	-	expression tag
Glu 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	91996.4
Non-Polymers*	Number of molecules	23
	Total formula weight	751.3
All*	Total formula weight	92747.7

*Water molecules are not included.