5HBB
Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG - E139A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.950, 63.488, 104.453 |
| Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
| Resolution | 111.800 - 2.470 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f44 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.800 | 2.554 |
| High resolution limit [Å] | 2.470 | 2.466 |
| Rmerge | 0.089 | 0.347 |
| Number of reflections | 44441 | |
| <I/σ(I)> | 17.36 | 3.84 |
| Completeness [%] | 83.5 | 27.02 |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M sodium thiocynate, 15% PEG3350 |
