5HAB
Crystal structure of mpy-RNase J (mutant H84A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Ribonuclease J | polymer | 470 | 52113.4 | 2 | UniProt (K4MAF9) In PDB | Methanolobus psychrophilus R15 | RNase J |
2 | C, D | RNA (5'-R(P*AP*AP*AP*AP*A)-3') | polymer | 5 | 1601.1 | 2 | synthetic construct | ||
3 | A, B | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
4 | water | water | 18.0 | 282 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 448 (UniProt: K4MAF9)
PDB | External Database | Details |
---|---|---|
Met -21 | - | initiating methionine |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
Ser -11 | - | expression tag |
Ser -10 | - | expression tag |
Gly -9 | - | expression tag |
Leu -8 | - | expression tag |
Val -7 | - | expression tag |
Pro -6 | - | expression tag |
Arg -5 | - | expression tag |
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
His -2 | - | expression tag |
Met -1 | - | expression tag |
Ala 0 | - | expression tag |
Ser 1 | - | expression tag |
Ala 84 | His 84 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 107428.9 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 864.6 | |
All* | Total formula weight | 108293.5 |