5HAB
Crystal structure of mpy-RNase J (mutant H84A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-18 |
Detector | PHILLIPS |
Wavelength(s) | 0.97845 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 169.680, 169.680, 167.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.210 - 2.300 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.937 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (DEV_1980) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.840 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.105 | 0.919 |
Number of reflections | 63078 | |
<I/σ(I)> | 13.1 | 2.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.6 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.8M ammonium sulfate, 3% glycerol |