5H7D

Crystal structure of the YgjG-protein A-Zpa963-calmodulin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F, I... (A, B, C, D, I...)	Putrescine aminotransferase,Immunoglobulin G-binding protein A	polymer	499	54225.1	8	UniProt (P42588) UniProt (P38507) Pfam (PF00202) Pfam (PF02216)	Escherichia coli (strain K12)	Putrescine--2-oxoglutaric acid transaminase,PATase,IgG-binding protein A,Staphylococcal protein A
2	C, D, G, H, K... (E, F, G, H, K...)	Zpa963,Calmodulin	polymer	120	13255.7	8	UniProt (O16305) Pfam (PF13499)	synthetic construct	CaM,CaM
3	AA, BA, CA, DA, EA... (L, O, P, E, F...)	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)

Sequence modifications

A, B, C, D, I, J, M, N: 7 - 453 (UniProt: P42588)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Met 6	-	expression tag

A, B, C, D, I, J, M, N: 454 - 501 (UniProt: P38507)

PDB	External Database	Details
Val 456	Asn 222	engineered mutation
Ala 474	Gly 240	engineered mutation

E, F, G, H, K, L, O, P: 100 - 156 (PDB: 5H7D)
E, F, G, H, K, L, O, P: 157 - 219 (UniProt: O16305)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	539847.0
Non-Polymers*	Number of molecules	16
	Total formula weight	641.2
All*	Total formula weight	540488.2

*Water molecules are not included.