5H7D
Crystal structure of the YgjG-protein A-Zpa963-calmodulin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.489, 155.376, 155.491 |
| Unit cell angles | 84.84, 89.68, 89.56 |
Refinement procedure
| Resolution | 50.000 - 2.570 |
| R-factor | 0.20754 |
| Rwork | 0.204 |
| R-free | 0.24092 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uoy 2m5a 3dve |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 210653 | |
| <I/σ(I)> | 14.6 | 1.2 |
| Completeness [%] | 90.5 | 83.4 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 296 | 0.1M HEPES pH 7.5, 20.7% PEG 300, 99mM calcium chloride |
