5H4R

the complex of Glycoside Hydrolase 5 Lichenase from Caldicellulosiruptor sp. F32 E188Q mutant and cellotetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-1,3-1,4-glucanase	polymer	396	45882.1	1	UniProt (R9RX81) Pfam (PF00150) In PDB	Caldicellulosiruptor sp. F32	Glycoside hydrolase F32EG5
2	B	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		666.6	1	In PDB BIRD (PRD_900011) GlyTouCan (G00025MO)		beta-cellotetraose
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	443	Chemie (HOH)

Sequence modifications

A: 19 - 396 (UniProt: R9RX81)

PDB	External Database	Details
Met 1	-	expression tag
Arg 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Met 12	-	expression tag
Ala 13	-	expression tag
Ser 14	-	expression tag
Leu 15	-	expression tag
Asn 16	-	expression tag
Arg 17	-	expression tag
Glu 18	-	expression tag
Gln 188	Glu 193	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45882.1
Branched	Number of molecules	1
	Total formula weight	666.6
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	46640.7

*Water molecules are not included.