5H4R
the complex of Glycoside Hydrolase 5 Lichenase from Caldicellulosiruptor sp. F32 E188Q mutant and cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.581, 76.315, 95.611 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.400 - 1.703 |
R-factor | 0.142735000316 |
Rwork | 0.141 |
R-free | 0.16984 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x0v |
RMSD bond length | 0.009 |
RMSD bond angle | 0.992 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.400 |
High resolution limit [Å] | 1.703 |
Number of reflections | 49925 |
<I/σ(I)> | 33.5 |
Completeness [%] | 98.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 291 | 0.2 M ammonium sulfate, 0.1 M Tris, 25% PEG3350 |