5H4R

the complex of Glycoside Hydrolase 5 Lichenase from Caldicellulosiruptor sp. F32 E188Q mutant and cellotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	62.581, 76.315, 95.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.400 - 1.703
R-factor	0.142735000316
Rwork	0.141
R-free	0.16984
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x0v
RMSD bond length	0.009
RMSD bond angle	0.992
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.400
High resolution limit [Å]	1.703
Number of reflections	49925
<I/σ(I)>	33.5
Completeness [%]	98.3
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	291	0.2 M ammonium sulfate, 0.1 M Tris, 25% PEG3350