5GY7
X-Ray structure of H243I mutant of UDP-Galactose 4-epimerase from E.coli:evidence for existence of open and closed active site during catalysis.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | UDP-glucose 4-epimerase | polymer | 344 | 38111.9 | 2 | UniProt (P09147) Pfam (PF01370) | Escherichia coli (strain K12) | Galactowaldenase,UDP-galactose 4-epimerase |
| 2 | C, F (A, B) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) | |||
| 3 | D, G (A, B) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 4 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | H (B) | NITRATE ION | non-polymer | 62.0 | 1 | Chemie (NO3) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 644 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 338 (UniProt: P09147)
| PDB | External Database | Details |
|---|---|---|
| Ile 243 | His 243 | engineered mutation |
| His 339 | - | expression tag |
| His 340 | - | expression tag |
| His 341 | - | expression tag |
| His 342 | - | expression tag |
| His 343 | - | expression tag |
| His 344 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 76223.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2289.3 | |
| All* | Total formula weight | 78513.1 |
