5GY7

X-Ray structure of H243I mutant of UDP-Galactose 4-epimerase from E.coli:evidence for existence of open and closed active site during catalysis.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	UDP-glucose 4-epimerase	polymer	344	38111.9	2	UniProt (P09147) Pfam (PF01370) In PDB	Escherichia coli (strain K12)	Galactowaldenase,UDP-galactose 4-epimerase
2	A, B	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
3	A, B	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	B	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
6		water	water		18.0	644	Chemie (HOH)

Sequence modifications

A, B: 1 - 338 (UniProt: P09147)

PDB	External Database	Details
Ile 243	His 243	engineered mutation
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76223.8
Non-Polymers*	Number of molecules	6
	Total formula weight	2289.3
All*	Total formula weight	78513.1

*Water molecules are not included.