5GY7
X-Ray structure of H243I mutant of UDP-Galactose 4-epimerase from E.coli:evidence for existence of open and closed active site during catalysis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-06 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.110, 91.160, 80.260 |
| Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
| Resolution | 79.070 - 1.430 |
| R-factor | 0.13517 |
| Rwork | 0.134 |
| R-free | 0.16014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xel |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.454 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.070 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 121857 |
| <I/σ(I)> | 7.73 |
| Completeness [%] | 98.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M Ammonium Nitrate, 20% PEG 3350 |
