5GWJ

Structure of a Human topoisomerase IIbeta fragment in complex with DNA and E7873S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA topoisomerase 2-beta	polymer	803	91928.4	2	UniProt (Q02880) Pfam (PF01751) Pfam (PF16898) Pfam (PF00521) In PDB	Homo sapiens (Human)	DNA topoisomerase II,beta isozyme
2	C, E	DNA (5'-D(P*AP*GP*CP*CP*GP*AP*GP*C)-3')	polymer	8	2436.6	2		synthetic construct
3	D, F	DNA (5'-D(P*TP*GP*CP*AP*GP*CP*TP*CP*GP*GP*CP*T)-3')	polymer	12	3654.4	2		synthetic construct
4	A, B	chloro{4,5-di(amino-kappaN)-N-[9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydro-2H-furo[3',4':6,7]naphtho[2,3-d][1,3]dioxol-5-yl]pentanamide}platinum	non-polymer		744.1	2	Chemie (N2S)
5	A, B	chloro{4,5-di(amino-kappaN)-N-[9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydro-2H-furo[3',4':6,7]naphtho[2,3-d][1,3]dioxol-5-yl]pentanamide}platinum	non-polymer		744.1	2	Chemie (N2W)
6	A, B, E, F	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7		water	water		18.0	312	Chemie (HOH)

Sequence modifications

A, B: 445 - 1201 (UniProt: Q02880)

PDB	External Database	Details
Met 419	-	expression tag
Ala 420	-	expression tag
Ser 421	-	expression tag
Trp 422	-	expression tag
Ser 423	-	expression tag
His 424	-	expression tag
Pro 425	-	expression tag
Gln 426	-	expression tag
Phe 427	-	expression tag
Glu 428	-	expression tag
Lys 429	-	expression tag
Gly 430	-	expression tag
Ala 431	-	expression tag
Asp 432	-	expression tag
Asp 433	-	expression tag
Asp 434	-	expression tag
Asp 435	-	expression tag
Lys 436	-	expression tag
Val 437	-	expression tag
Pro 438	-	expression tag
Asp 439	-	expression tag
Pro 440	-	expression tag
Thr 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Gly 1202	-	expression tag
Ala 1203	-	expression tag
Pro 1204	-	expression tag
Gly 1205	-	expression tag
Phe 1206	-	expression tag
Ser 1207	-	expression tag
Ser 1208	-	expression tag
Ile 1209	-	expression tag
Ser 1210	-	expression tag
Ala 1211	-	expression tag
His 1212	-	expression tag
His 1213	-	expression tag
His 1214	-	expression tag
His 1215	-	expression tag
His 1216	-	expression tag
His 1217	-	expression tag
His 1218	-	expression tag
His 1219	-	expression tag
His 1220	-	expression tag
His 1221	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	196038.7
Non-Polymers*	Number of molecules	10
	Total formula weight	3122.1
All*	Total formula weight	199160.8

*Water molecules are not included.