5GR2

Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	1,4-alpha-glucan branching enzyme GlgB	polymer	793	92644.3	1	UniProt (B1WPM8) Pfam (PF22019) Pfam (PF02922) Pfam (PF00128) Pfam (PF02806)	Cyanothece sp. (strain ATCC 51142)	1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase,Alpha-(1->4)-glucan branching enzyme,Glycogen branching enzyme
2	B, C, D, E, F (A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
3	G, H, I, J (A)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	K (A)	water	water		18.0	767	Chemie (HOH)

Sequence modifications

A: 1 - 773 (UniProt: B1WPM8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 541	Leu 541	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	92644.3
Non-Polymers*	Number of molecules	9
	Total formula weight	557.7
All*	Total formula weight	93202.0

*Water molecules are not included.