5GR2
Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 1,4-alpha-glucan branching enzyme GlgB | polymer | 793 | 92644.3 | 1 | UniProt (B1WPM8) Pfam (PF22019) Pfam (PF02922) Pfam (PF00128) Pfam (PF02806) In PDB | Cyanothece sp. (strain ATCC 51142) | 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase,Alpha-(1->4)-glucan branching enzyme,Glycogen branching enzyme |
2 | A | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
3 | A | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
4 | water | water | 18.0 | 767 | Chemie (HOH) |
Sequence modifications
A: 1 - 773 (UniProt: B1WPM8)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 541 | Leu 541 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 92644.3 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 557.7 | |
All* | Total formula weight | 93202.0 |