5GR2
Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.483, 133.483, 184.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 1.950 |
| R-factor | 0.15516 |
| Rwork | 0.154 |
| R-free | 0.17954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gqu |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.146 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.040 | 0.247 |
| Number of reflections | 121552 | |
| <I/σ(I)> | 59.4 | 11.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | magnesium chloride, ethanol, HEPES-NaOH |
