5GQH

Cryo-EM structure of PaeCas3-AcrF3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST	polymer	1090	122892.4	1	UniProt (Q02ML8) Pfam (PF21384) Pfam (PF18019) Pfam (PF22590) Pfam (PF21802)	Pseudomonas aeruginosa UCBPP-PA14	type I-F CRISPR-associated nuclease/helicase Cas3
2	B, C (B, C)	anti-CRISPR protein 3	polymer	139	15806.5	2	UniProt (L7P7R7) Pfam (PF21401)	Pseudomonas phage JBD5	AcrF3,Uncharacterized protein

Sequence modifications

A: 1 - 1076 (UniProt: Q02ML8)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	154505.4
All*	Total formula weight	154505.4

*Water molecules are not included.