5GPQ

Crystal Structure of zebrafish ASC CARD Domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein,Apoptosis-associated speck-like protein containing a CARD	polymer	467	51654.5	1	UniProt (P0AEY0) UniProt (Q9I9N6) Pfam (PF01547) Pfam (PF00619) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein,PYD and CARD domain-containing protein
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
4		water	water		18.0	309	Chemie (HOH)

Sequence modifications

A: 2 - 359 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	expression tag
Ala 83	Asp 108	engineered mutation
Ala 84	Lys 109	engineered mutation
Ala 173	Glu 198	engineered mutation
Ala 174	Asn 199	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 360	-	linker
Ala 361	-	linker
Leu 362	-	linker
Ala 363	-	linker
Ala 364	-	linker
Ala 365	-	linker
Gln 366	-	linker
Thr 367	-	linker
Asn 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
Arg 371	-	linker
Ala 372	-	linker
Phe 373	-	linker
Ala 374	-	linker
Ala 375	-	linker
Ala 376	-	linker

A: 377 - 459 (UniProt: Q9I9N6)

PDB	External Database	Details
Leu 460	-	expression tag
Glu 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51654.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	1
	Total formula weight	192.1
All*	Total formula weight	52188.9

*Water molecules are not included.