5GPQ
Crystal Structure of zebrafish ASC CARD Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 190 |
Detector technology | CCD |
Collection date | 2013-03-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0331 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.260, 48.411, 101.964 |
Unit cell angles | 90.00, 124.24, 90.00 |
Refinement procedure
Resolution | 43.564 - 2.100 |
R-factor | 0.1611 |
Rwork | 0.159 |
R-free | 0.19940 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 239603 |
<I/σ(I)> | 14 |
Completeness [%] | 99.8 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.0 M Ammonium Citrate, 0.1 M HEPES |