5GPQ

Crystal Structure of zebrafish ASC CARD Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	190
Detector technology	CCD
Collection date	2013-03-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	139.260, 48.411, 101.964
Unit cell angles	90.00, 124.24, 90.00

Refinement procedure

Resolution	43.564 - 2.100
R-factor	0.1611
Rwork	0.159
R-free	0.19940
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	239603
<I/σ(I)>	14
Completeness [%]	99.8
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2.0 M Ammonium Citrate, 0.1 M HEPES