5GNM

Cytochrome P450 Vdh (CYP107BR1) L348M mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Vitamin D(3) 25-hydroxylase	polymer	411	45505.6	4	UniProt (C4B644) Pfam (PF00067) In PDB	Pseudonocardia autotrophica (Amycolata autotrophica)	Cytochrome P450, CYP107BR1
2	A, B, C, D	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
3		water	water		18.0	132	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 403 (UniProt: C4B644)

PDB	External Database	Details
Met 348	Leu 348	engineered mutation
Leu 404	-	expression tag
Glu 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	182022.6
Non-Polymers*	Number of molecules	4
	Total formula weight	2465.9
All*	Total formula weight	184488.5

*Water molecules are not included.