5GLM

Crystal structure of CoXyl43, GH43 beta-xylosidase/alpha-arabinofuranosidase from a compost microbial metagenome in complex with xylotriose, calcium-free form.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase family 43	polymer	344	38529.5	2	UniProt (A0A0H5BL38) Pfam (PF04616)	uncultured bacterium	beta-xylosidase / alpha-arabinofuranosidase
2	C, D, E (C, D, E)	beta-D-xylopyranose-(1-4)-beta-D-xylopyranose	branched		282.2	3	In PDB BIRD (PRD_900116) GlyTouCan (G87728WL)		4beta-beta-xylobiose
3	F, G (F, G)	beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose	branched		414.4	2	In PDB BIRD (PRD_900117) GlyTouCan (G87429QT)		4beta-beta-xylotriose
4	H, I, L, M (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	J, N (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
6	K, P (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
7	O (B)	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
8	Q, R (A, B)	water	water		18.0	634	Chemie (HOH)

Sequence modifications

A, B: 47 - 369 (UniProt: A0A0H5BL38)

PDB	External Database	Details
Met 26	-	expression tag
Gly 27	-	expression tag
Ser 28	-	expression tag
Ser 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
Ser 36	-	expression tag
Ser 37	-	expression tag
Gly 38	-	expression tag
Leu 39	-	expression tag
Val 40	-	expression tag
Pro 41	-	expression tag
Arg 42	-	expression tag
Gly 43	-	expression tag
Ser 44	-	expression tag
His 45	-	expression tag
Met 46	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77059.0
Branched	Number of molecules	5
	Total formula weight	1675.5
Non-Polymers*	Number of molecules	9
	Total formula weight	572.4
All*	Total formula weight	79306.9

*Water molecules are not included.