5GLM
Crystal structure of CoXyl43, GH43 beta-xylosidase/alpha-arabinofuranosidase from a compost microbial metagenome in complex with xylotriose, calcium-free form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-05-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.774, 61.647, 79.006 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 78.610 - 1.800 |
R-factor | 0.14667 |
Rwork | 0.145 |
R-free | 0.16869 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5glk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.064 | 0.241 |
Number of reflections | 66344 | |
<I/σ(I)> | 31.6 | 9.3 |
Completeness [%] | 99.8 | 99 |
Redundancy | 7.5 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% PEG 3350, 8% Tacsimate |