5GIO

Crystal structure of box C/D RNP with 12 nt guide regions and 13 nt substrates

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, M (A, B, K)	C/D box methylation guide ribonucleoprotein complex aNOP56 subunit	polymer	388	44168.5	3	UniProt (A0A0E3MJI1) Pfam (PF21572) Pfam (PF01798) UniProt (by SIFTS) (Q97ZH3)	Sulfolobus solfataricus
2	B, E, N (C, D, L)	50S ribosomal protein L7Ae	polymer	130	14075.3	3	UniProt (A0A0E3JZF7) Pfam (PF01248) UniProt (by SIFTS) (P55858)	Sulfolobus solfataricus	Ribosomal protein L8e
3	C, F, O (E, F, M)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26439.4	3	UniProt (A0A0E3JUC9) Pfam (PF01269) UniProt (by SIFTS) (P58032)	Sulfolobus solfataricus
4	G, H, K (G, H, N)	C/D RNA	polymer	40	12852.7	3		Sulfolobus solfataricus
5	I, J, L (I, J, O)	substrate	polymer	13	4181.6	3		Sulfolobus solfataricus
6	P, Q, R (E, F, M)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	3	Chemie (SAH)

Sequence modifications

A, B, K: 4 - 380 (UniProt: A0A0E3MJI1)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 2	-	expression tag
Lys 3	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

C, D, L: 6 - 130 (UniProt: A0A0E3JZF7)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Ser 5	-	expression tag

E, F, M: 3 - 232 (UniProt: A0A0E3JUC9)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	305152.2
Non-Polymers*	Number of molecules	3
	Total formula weight	1153.2
All*	Total formula weight	306305.5

*Water molecules are not included.