5GIN

Crystal structure of box C/D RNP with 12 nt guide regions and 9 nt substrates

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, M (A, B, K)	C/D box methylation guide ribonucleoprotein complex aNOP56 subunit	polymer	388	44168.5	3	UniProt (A0A0E3MJI1) Pfam (PF21572) Pfam (PF01798) UniProt (by SIFTS) (Q97ZH3)	Sulfolobus solfataricus
2	B, E, N (C, D, L)	50S ribosomal protein L7Ae	polymer	130	14075.3	3	UniProt (A0A0E3JZF7) Pfam (PF01248) UniProt (by SIFTS) (P55858)	Sulfolobus solfataricus	Ribosomal protein L8e
3	C, F, O (E, F, M)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26439.4	3	UniProt (A0A0E3JUC9) Pfam (PF01269) UniProt (by SIFTS) (P58032)	Sulfolobus solfataricus
4	G, H, K (G, H, N)	C/D RNA	polymer	40	12852.7	3		Sulfolobus solfataricus
5	I, J, L (I, J, O)	substrate	polymer	9	2871.8	3		Sulfolobus solfataricus
6	P, Q, R (E, F, M)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	3	Chemie (SAH)

Sequence modifications

A, B, K: 4 - 380 (UniProt: A0A0E3MJI1)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 2	-	expression tag
Lys 3	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

C, D, L: 6 - 130 (UniProt: A0A0E3JZF7)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Ser 5	-	expression tag

E, F, M: 3 - 232 (UniProt: A0A0E3JUC9)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	301222.9
Non-Polymers*	Number of molecules	3
	Total formula weight	1153.2
All*	Total formula weight	302376.1

*Water molecules are not included.