5GIN

Crystal structure of box C/D RNP with 12 nt guide regions and 9 nt substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	242.676, 242.676, 146.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.999 - 3.308
R-factor	0.2531
Rwork	0.250
R-free	0.30310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pla
RMSD bond length	0.013
RMSD bond angle	1.711
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.131
Number of reflections	63174
<I/σ(I)>	12
Completeness [%]	96.5
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.0 M ammonium sulfate, 2% v/v PEG 400, 10 mM magnesium chloride, 0.1 M HEPES