5G6T

Crystal structure of Zn-containing NagZ H174A mutant from Pseudomonas aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	BETA-HEXOSAMINIDASE	polymer	352	38250.6	2	UniProt (Q9HZK0) Pfam (PF00933)	PSEUDOMONAS AERUGINOSA	BETA-N-ACETYLHEXOSAMINIDASE, N-ACETYL-BETA-GLUCOSAMINIDASE, NAGZ
2	C, E (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	F, G (A, B)	water	water		18.0	393	Chemie (HOH)

Sequence modifications

A, B: 1 - 332 (UniProt: Q9HZK0)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 174	His 174	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76501.2
Non-Polymers*	Number of molecules	3
	Total formula weight	236.9
All*	Total formula weight	76738.1

*Water molecules are not included.