5G6T
Crystal structure of Zn-containing NagZ H174A mutant from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.095, 76.122, 76.005 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 44.937 - 2.150 |
| R-factor | 0.1816 |
| Rwork | 0.178 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE OF NAGZ FROM PSEUDOMONAS AERUGINOSA |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.180 | 0.950 |
| Number of reflections | 36891 | |
| <I/σ(I)> | 8.3 | 1.6 |
| Completeness [%] | 97.2 | 97.3 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 30% PEG 8000 100 MM SODIUM CACODYLATE PH 6.0 200 MM SODIUM ACETATE PH 6.0 |
