5G3P

Bacillus cereus formamidase (BceAmiF) acetylated at the active site.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	FORMAMIDASE	polymer	346	38715.8	6	UniProt (E5LR94) Pfam (PF00795) UniProt (by SIFTS) (P59701) In PDB	BACILLUS CEREUS	BCEAMIF, FORMAMIDE AMIDOHYDROLASE
2	D, A, E, B, F	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	12	Chemie (PEG)
3	A, E, C	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4	A, B, F, C	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	C, A, E, B, F	SODIUM ION	non-polymer		23.0	15	Chemie (NA)
6	D, E, B, F, C	TRIETHYLENE GLYCOL	non-polymer		150.2	6	Chemie (PGE)
7	B	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8	B, F	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
9		water	water		18.0	1938	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 332 (UniProt: E5LR94)

PDB	External Database	Details
Trp 333	-	expression tag
Phe 334	-	expression tag
Arg 335	-	expression tag
Val 336	-	expression tag
Asp 337	-	expression tag
Pro 338	-	expression tag
Leu 339	-	expression tag
Glu 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	232294.6
Non-Polymers*	Number of molecules	43
	Total formula weight	3325.9
All*	Total formula weight	235620.4

*Water molecules are not included.