5G3P
Bacillus cereus formamidase (BceAmiF) acetylated at the active site.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004328 | molecular_function | formamidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| A | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| B | 0004328 | molecular_function | formamidase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| B | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| C | 0004328 | molecular_function | formamidase activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| C | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| D | 0004328 | molecular_function | formamidase activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| D | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| E | 0004328 | molecular_function | formamidase activity |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| E | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| F | 0004328 | molecular_function | formamidase activity |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| F | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PEG A 1329 |
| Chain | Residue |
| A | ASN293 |
| A | PRO294 |
| A | HOH2323 |
| A | HOH2377 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PEG A 1330 |
| Chain | Residue |
| A | HOH2110 |
| A | THR68 |
| A | LYS69 |
| A | TYR322 |
| A | GLY323 |
| A | TYR324 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE EDO A 1331 |
| Chain | Residue |
| A | GLN233 |
| A | THR241 |
| A | GLN244 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1332 |
| Chain | Residue |
| A | PRO309 |
| A | TRP310 |
| A | GLU311 |
| A | ASP312 |
| A | GLU313 |
| A | HOH2344 |
| site_id | AC5 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE PEG B 1328 |
| Chain | Residue |
| B | VAL243 |
| B | GLN244 |
| B | GLY245 |
| B | GLU251 |
| B | ILE252 |
| B | VAL253 |
| B | NA1335 |
| B | HOH2256 |
| B | HOH2326 |
| E | THR193 |
| E | SER196 |
| E | GLU197 |
| E | ARG247 |
| E | HOH2197 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PEG B 1329 |
| Chain | Residue |
| B | THR68 |
| B | LYS69 |
| B | TYR322 |
| B | TYR324 |
| B | HOH2124 |
| B | HOH2325 |
| C | SER48 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGE B 1330 |
| Chain | Residue |
| B | VAL80 |
| B | MET126 |
| B | LYS129 |
| B | HOH2145 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1331 |
| Chain | Residue |
| A | ASP146 |
| B | ASN293 |
| B | PRO294 |
| B | LYS299 |
| B | HOH2291 |
| B | HOH2293 |
| B | HOH2327 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 1332 |
| Chain | Residue |
| B | HIS246 |
| B | ARG247 |
| B | ASN248 |
| C | HIS246 |
| C | ARG247 |
| C | ASN248 |
| E | HIS246 |
| E | ARG247 |
| E | ASN248 |
| site_id | BC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 1333 |
| Chain | Residue |
| B | GLY82 |
| B | ASP86 |
| site_id | BC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGE C 1327 |
| Chain | Residue |
| C | THR68 |
| C | LYS69 |
| C | TYR322 |
| C | TYR324 |
| C | HOH2030 |
| C | HOH2349 |
| E | SER48 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGE C 1328 |
| Chain | Residue |
| C | ASN293 |
| C | PRO294 |
| C | LYS299 |
| C | HOH2299 |
| site_id | BC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE EDO C 1329 |
| Chain | Residue |
| E | GLN38 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT C 1330 |
| Chain | Residue |
| C | GLY82 |
| C | ASP86 |
| C | HOH2121 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PEG D 1328 |
| Chain | Residue |
| A | TRP250 |
| A | HOH2077 |
| D | ASP223 |
| D | GLY224 |
| site_id | BC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PEG D 1329 |
| Chain | Residue |
| D | PRO294 |
| D | LYS299 |
| site_id | BC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PEG D 1330 |
| Chain | Residue |
| D | VAL243 |
| D | GLN244 |
| D | GLY245 |
| D | GLU251 |
| D | ILE252 |
| D | VAL253 |
| D | HOH2302 |
| D | HOH2303 |
| F | THR193 |
| F | SER196 |
| F | GLU197 |
| F | ARG247 |
| site_id | BC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGE D 1331 |
| Chain | Residue |
| A | SER48 |
| D | THR68 |
| D | LYS69 |
| D | TYR322 |
| site_id | CC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PEG E 1327 |
| Chain | Residue |
| E | THR68 |
| E | LYS69 |
| E | TYR322 |
| E | TYR324 |
| E | HOH2309 |
| site_id | CC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG E 1328 |
| Chain | Residue |
| E | PRO83 |
| E | ASP86 |
| E | HOH2091 |
| site_id | CC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PGE E 1329 |
| Chain | Residue |
| E | ASN293 |
| E | PRO294 |
| E | LYS299 |
| site_id | CC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO E 1330 |
| Chain | Residue |
| E | ARG204 |
| E | GLN233 |
| E | THR241 |
| E | GLN244 |
| E | HOH2202 |
| E | HOH2314 |
| E | HOH2315 |
| E | HOH2316 |
| site_id | CC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG F 1327 |
| Chain | Residue |
| F | ASN293 |
| F | PRO294 |
| F | LYS299 |
| site_id | CC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PEG F 1328 |
| Chain | Residue |
| A | THR193 |
| A | SER196 |
| A | GLU197 |
| A | ARG247 |
| F | VAL243 |
| F | GLN244 |
| F | GLU251 |
| F | ILE252 |
| F | VAL253 |
| F | NA1335 |
| F | HOH2198 |
| site_id | CC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG F 1329 |
| Chain | Residue |
| F | VAL25 |
| F | ASP223 |
| F | HOH2261 |
| site_id | CC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGE F 1330 |
| Chain | Residue |
| D | SER48 |
| F | THR68 |
| F | LYS69 |
| F | TYR322 |
| F | GLY323 |
| site_id | CC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 F 1331 |
| Chain | Residue |
| A | HIS246 |
| A | ARG247 |
| A | ASN248 |
| D | HIS246 |
| D | ARG247 |
| D | ASN248 |
| F | HIS246 |
| F | ARG247 |
| F | ASN248 |
| site_id | DC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT F 1332 |
| Chain | Residue |
| F | PRO309 |
| F | TRP310 |
| F | GLU311 |
| F | ASP312 |
| F | GLU313 |
| F | NA1333 |
| site_id | DC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE NA A 1340 |
| Chain | Residue |
| C | HOH2189 |
| D | GLU292 |
| site_id | DC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA F 1333 |
| Chain | Residue |
| F | LYS307 |
| F | VAL308 |
| F | PRO309 |
| F | ACT1332 |
| site_id | DC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 1331 |
| Chain | Residue |
| B | GLN194 |
| B | HOH2232 |
| C | SER4 |
| C | GLY5 |
| site_id | DC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA F 1334 |
| Chain | Residue |
| F | HIS44 |
| F | SER48 |
| F | HOH2059 |
| F | HOH2070 |
| site_id | DC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA B 1334 |
| Chain | Residue |
| B | SER4 |
| B | GLY5 |
| B | HOH2004 |
| E | GLN194 |
| site_id | DC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA F 1335 |
| Chain | Residue |
| A | THR193 |
| A | HOH2241 |
| A | HOH2244 |
| F | THR254 |
| F | PEG1328 |
| site_id | DC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA E 1340 |
| Chain | Residue |
| E | HOH2166 |
| E | HOH2167 |
| F | HOH2222 |
| site_id | DC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA B 1335 |
| Chain | Residue |
| B | THR254 |
| B | PEG1328 |
| B | HOH2002 |
| B | HOH2106 |
| E | THR193 |
| site_id | EC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 1332 |
| Chain | Residue |
| C | SER205 |
| C | ASN206 |
| C | GLN209 |
| D | GLN209 |
| site_id | EC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA F 1336 |
| Chain | Residue |
| E | ASN134 |
| E | HOH2158 |
| F | ASN277 |
| F | ARG281 |
| site_id | EC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA B 1336 |
| Chain | Residue |
| B | THR79 |
| B | VAL80 |
| B | GLU105 |
| B | HOH2145 |
| B | HOH2174 |
| site_id | EC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA E 1331 |
| Chain | Residue |
| E | PRO171 |
| E | GLU172 |
| E | HOH2186 |
| F | GLU172 |
| F | PHE297 |
| site_id | EC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA B 1337 |
| Chain | Residue |
| B | THR73 |
| B | GLU75 |
| B | HOH2328 |
| site_id | EC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA E 1332 |
| Chain | Residue |
| E | SER12 |
| E | GLY156 |
| E | GLU260 |
| E | HOH2009 |
| site_id | EC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE NA A 1333 |
| Chain | Residue |
| A | ASN293 |
| B | ALA302 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01243 |
| Chain | Residue | Details |
| A | GLU60 | |
| B | GLU60 | |
| C | GLU60 | |
| D | GLU60 | |
| E | GLU60 | |
| F | GLU60 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01243 |
| Chain | Residue | Details |
| A | LYS132 | |
| B | LYS132 | |
| C | LYS132 | |
| D | LYS132 | |
| E | LYS132 | |
| F | LYS132 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_01243 |
| Chain | Residue | Details |
| A | SCY165 | |
| B | SCY165 | |
| C | SCY165 | |
| D | SCY165 | |
| E | SCY165 | |
| F | SCY165 |
