5FW3
Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | SGRNA | polymer | 83 | 26789.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 2 | B (B) | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 | polymer | 1372 | 158987.2 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | STREPTOCOCCUS PYOGENES | CAS9 VRER VARIANT, SPCAS9 |
| 3 | C (C) | TARGET DNA STRAND | polymer | 28 | 8553.6 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | D (D) | NON-TARGET DNA STRAND | polymer | 12 | 3710.5 | 1 | SYNTHETIC CONSTRUCT | ||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I, J, K... (A, B) | POTASSIUM ION | non-polymer | 39.1 | 11 | Chemie (K) | |||
| 7 | R, S, T (A, B, C) | water | water | 18.0 | 152 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| Val 1135 | Asp 1135 | engineered mutation |
| Arg 1218 | Gly 1218 | engineered mutation |
| Glu 1335 | Arg 1335 | engineered mutation |
| Arg 1337 | Thr 1337 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 198040.2 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 478.7 | |
| All* | Total formula weight | 198518.9 |
