5FVF
Room temperature structure of IrisFP determined by serial femtosecond crystallography.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Green to red photoconvertible GFP-like protein EosFP | polymer | 225 | 25605.0 | 4 | UniProt (Q5S6Z9) Pfam (PF01353) | Lobophyllia hemprichii (Lobed brain coral) | |
| 2 | E, F, G, H, K... (A, B, C, D) | SULFATE ION | non-polymer | 96.1 | 19 | Chemie (SO4) | |||
| 3 | I, J (A) | AMMONIUM ION | non-polymer | 18.0 | 2 | Chemie (NH4) | |||
| 4 | AA, BA, CA, Z (B, C, D, A) | water | water | 18.0 | 344 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 223 (UniProt: Q5S6Z9)
| PDB | External Database | Details |
|---|---|---|
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| 5sq 64 | His 62 | chromophore |
| 5sq 64 | Tyr 63 | chromophore |
| 5sq 64 | Gly 64 | chromophore |
| Ser 173 | Phe 173 | engineered mutation |
| Leu 191 | Phe 191 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 102419.9 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 1861.3 | |
| All* | Total formula weight | 104281.2 |
