5FVF
Room temperature structure of IrisFP determined by serial femtosecond crystallography.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 297 |
Detector technology | CCD |
Collection date | 2014-02-14 |
Detector | MPCCD |
Wavelength(s) | 1.77 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.330, 97.760, 142.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.241 - 2.750 |
R-factor | 0.182 |
Rwork | 0.176 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vvh |
RMSD bond length | 0.014 |
RMSD bond angle | 0.661 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.220 | 1.000 |
Number of reflections | 30981 | |
<I/σ(I)> | 3.39 | 0.95 |
Completeness [%] | 100.0 | 100 |
Redundancy | 159 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3 M AMMONIUM SULFATE, 100MM BICINE, PH 8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |