5FUC

Biophysical and cellular characterisation of a junctional epitope antibody that locks IL-6 and gp80 together in a stable complex: implications for new therapeutic strategies

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	INTERLEUKIN-6	polymer	166	18982.7	2	UniProt (P05231) Pfam (PF00489)	HOMO SAPIENS (HUMAN)	IL-6, B-CELL STIMULATORY FACTOR 2, BSF-2, IFN-BETA-2, CTL DIFFERENTIATION FACTOR, CDF, HYBRIDOMA GROWTH FACTOR, INTERFERON BETA-2
2	C, D (C, D)	INTERLEUKIN-6 RECEPTOR SUBUNIT ALPHA, INTERLEUKIN-6 RECEPTOR	polymer	231	25973.1	2	UniProt (D6R9R8) UniProt (P08887) Pfam (PF09240)	HOMO SAPIENS (HUMAN)	IL-6RA, IL-6R 1, MEMBRANE GLYCOPROTEIN 80, GP80
3	E, F (E, V)	VHH6	polymer	132	14677.2	2		CAMELUS DROMEDARIUS (CAMEL)
4	G, H, I, J (C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	111	Chemie (HOH)

Sequence modifications

A, B: 21 - 184 (UniProt: P05231)

PDB	External Database	Details
Gly 19	-	expression tag
Ser 20	-	expression tag

C, D: 74 - 87 (UniProt: D6R9R8)

PDB	External Database	Details
Gly 73	-	expression tag
Ala 88	-	linker
Gly 89	-	linker
Ala 90	-	linker
Gly 91	-	linker

C, D: 92 - 303 (UniProt: P08887)

PDB	External Database	Details
Ala 192	Cys 211	engineered mutation
Ala 258	Cys 277	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	119265.9
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	120150.7

*Water molecules are not included.