5FT8

Crystal structure of the complex between the cysteine desulfurase CsdA and the sulfur-acceptor CsdE in the persulfurated state at 2.50 Angstroem resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Cysteine desulfurase CsdA	polymer	403	43434.2	8	UniProt (Q46925) Pfam (PF00266)	Escherichia coli K-12	Cysteine sulfinate desulfinase,CSD,Selenocysteine lyase
2	B, D, F, H, J... (Q, S, U, W, Y...)	Sulfur acceptor protein CsdE	polymer	154	16946.3	8	UniProt (P0AGF2) Pfam (PF02657)	Escherichia coli K-12
3	AB, BC, IC, KA, KC... (E, I, K, C, M...)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	8	Chemie (PLP)
4	BB, CB, CC, DB, EB... (E, I, Q, K, C...)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	16	Chemie (PEG)
5	AA, AC, BA, CA, DA... (A, W, I, E, Q...)	GLYCEROL	non-polymer		92.1	51	Chemie (GOL)
6	AD, BD, NC, OC, PC... (c, O, A, Q, C...)	water	water		18.0	1021	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O: 1 - 401 (UniProt: Q46925)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Q, S, U, W, Y, a, c, e: 1 - 147 (UniProt: P0AGF2)

PDB	External Database	Details
Lys 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	483044.1
Non-Polymers*	Number of molecules	75
	Total formula weight	8371.9
All*	Total formula weight	491415.9

*Water molecules are not included.