5FNV

a new complex structure of tubulin with an alpha-beta unsaturated lactone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	TUBULIN ALPHA-1B CHAIN	polymer	451	50204.4	2	UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953)	GALLUS GALLUS (CHICKEN)	ALPHA-TUBULIN UBIQUITOUS, TUBULIN K-ALPHA-1, TUBULIN ALPHA- UBIQUITOUS CHAIN, TUBULIN ALPHA SUBUNIT
2	B, D (B, D)	TUBULIN BETA CHAIN	polymer	445	49907.8	2	UniProt (P02554) Pfam (PF00091) Pfam (PF03953)	RATTUS NORVEGICUS (NORWAY RAT)	BETA-TUBULIN, TUBULIN BETA SUBUNIT
3	E (E)	STATHMIN-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836)	SUS SCROFA (PIG)	STATHMIN-LIKE PROTEIN B3, RB3, ISTATHMIN-4
4	F (F)	TUBULIN TYROSINE LIGASE	polymer	384	44378.5	1	UniProt (E1BQ43)	SUS SCROFA (PIG)
5	G, O, S (A, C, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP)
6	H, K, P, T (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	I, L, Q (A, B, C)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
8	J (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
9	M, N (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
10	R (C)	PIRONETIN	non-polymer		326.5	1	Chemie (X3H)
11	U (F)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
12	V, W, X, Y, Z (A, B, C, D, F)	water	water		18.0	68	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	expression tag
Ala 4	-	expression tag

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261447.1
Non-Polymers*	Number of molecules	15
	Total formula weight	3452.3
All*	Total formula weight	264899.4

*Water molecules are not included.