5FMZ

Crystal structure of Influenza B polymerase with bound 5' vRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	POLYMERASE ACIDIC PROTEIN	polymer	751	85822.8	2	UniProt (Q5V8Z9) Pfam (PF00603)	INFLUENZA B VIRUS (B/MEMPHIS/13/2003)	INFLUENZA B POLYMERASE PA SUBUNIT
2	B, E (B, E)	RNA-DIRECTED RNA POLYMERASE CATALYTIC SUBUNIT	polymer	772	86207.0	2	UniProt (Q5V8Y6) Pfam (PF00602)	INFLUENZA B VIRUS (B/MEMPHIS/13/2003)	INFLUENZA B POLYMERASE PB1 SUBUNIT
3	C, F (C, F)	POLYMERASE BASIC PROTEIN 2	polymer	798	90844.1	2	UniProt (Q5V8X3) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	INFLUENZA B VIRUS (B/MEMPHIS/13/2003)	INFLUENZA B POLYMERASE PB2 SUBUNIT
4	G, H (H, V)	5'-R(*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP)-3'	polymer	12	3883.4	2		INFLUENZA B VIRUS (B/MEMPHIS/13/2003)	VRNA 5' END

Sequence modifications

A, D: 1 - 726 (UniProt: Q5V8Z9)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 727	-	expression tag
Ser 728	-	expression tag
Gly 729	-	expression tag
Ser 730	-	expression tag
Gly 731	-	expression tag
Glu 732	-	expression tag
Asn 733	-	expression tag
Leu 734	-	expression tag
Tyr 735	-	expression tag
Phe 736	-	expression tag
Gln 737	-	expression tag

B, E: 1 - 752 (UniProt: Q5V8Y6)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Glu 758	-	expression tag
Asn 759	-	expression tag
Leu 760	-	expression tag
Tyr 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

C, F: 1 - 770 (UniProt: Q5V8X3)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 771	-	expression tag
Trp 772	-	expression tag
Ser 773	-	expression tag
His 774	-	expression tag
Pro 775	-	expression tag
Gln 776	-	expression tag
Phe 777	-	expression tag
Glu 778	-	expression tag
Lys 779	-	expression tag
Gly 780	-	expression tag
Ser 781	-	expression tag
Gly 782	-	expression tag
Ser 783	-	expression tag
Glu 784	-	expression tag
Asn 785	-	expression tag
Leu 786	-	expression tag
Tyr 787	-	expression tag
Phe 788	-	expression tag
Gln 789	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	533514.6
All*	Total formula weight	533514.6

*Water molecules are not included.