5FMZ
Crystal structure of Influenza B polymerase with bound 5' vRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.570, 200.540, 133.130 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 126.830 - 3.400 |
| R-factor | 0.25595 |
| Rwork | 0.255 |
| R-free | 0.27696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wsa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.550 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.260 | 1.380 |
| Number of reflections | 86591 | |
| <I/σ(I)> | 7.83 | 1.66 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.37 | 6.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M KCL, 10 MM MGCL2, 50 MM TRIS-HCL PH 8.5 AND 30% PEG400 |
