5FKW
cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA POLYMERASE III ALPHA | polymer | 1160 | 130088.4 | 1 | UniProt (P10443) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) Pfam (PF01336) Pfam (PF20914) In PDB | ESCHERICHIA COLI K-12 | |
| 2 | B, C | DNA POLYMERASE III BETA | polymer | 366 | 40630.5 | 2 | UniProt (P0A988) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) In PDB | ESCHERICHIA COLI K-12 | |
| 3 | D | DNA POLYMERASE III EPSILON | polymer | 243 | 27119.0 | 1 | UniProt (P03007) Pfam (PF00929) In PDB | ESCHERICHIA COLI K-12 | |
| 4 | P | PRIMER-TEMPLATE DUPLEX DNA | polymer | 25 | 7797.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 5 | T | PRIMER-TEMPLATE DUPLEX DNA | polymer | 29 | 8796.7 | 1 | SYNTHETIC CONSTRUCT |
Sequence modifications
A: 1 - 1160 (UniProt: P10443)
D: 1 - 243 (UniProt: P03007)
| PDB | External Database | Details |
|---|---|---|
| Leu 921 | Ala 921 | engineered mutation |
| Leu 923 | Met 923 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 183 | Thr 183 | engineered mutation |
| Leu 185 | Met 185 | engineered mutation |
| Pro 186 | Ala 186 | engineered mutation |
| Leu 187 | Phe 187 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 255062.1 | |
| All* | Total formula weight | 255062.1 |
