5FKW
cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 QUANTUM (4k x 4k) |
Number of Images | 1350 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 7.3 |
Symmetry | C1 |