5FIO

DARPins as a new tool for experimental phasing in protein crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NI3C DARPIN MUTANT5 HG-SITE N1	polymer	172	18583.8	1		SYNTHETIC CONSTRUCT
2	B	MALTOSE-BINDING PERIPLASMIC PROTEIN	polymer	395	43187.7	1	UniProt (P0AEY0) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	ESCHERICHIA COLI	MBP, MMBP, MALTODEXTRIN-BINDING PROTEIN
3	A	MERCURY (II) ION	non-polymer		200.6	1	Chemie (HG)
4		water	water		18.0	202	Chemie (HOH)

Sequence modifications

B: 3 - 366 (UniProt: P0AEY0)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Lys 1	-	expression tag
Thr 2	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Thr 371	-	expression tag
Pro 372	-	expression tag
Gly 373	-	expression tag
Arg 374	-	expression tag
Pro 375	-	expression tag
Ala 376	-	expression tag
Ala 377	-	expression tag
Lys 378	-	expression tag
Leu 379	-	expression tag
Asn 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61771.6
Non-Polymers*	Number of molecules	1
	Total formula weight	200.6
All*	Total formula weight	61972.1

*Water molecules are not included.