5FIO
DARPins as a new tool for experimental phasing in protein crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.610, 43.790, 82.420 |
| Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
| Resolution | 45.178 - 2.100 |
| R-factor | 0.2133 |
| Rwork | 0.209 |
| R-free | 0.25250 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.566 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.550 |
| Number of reflections | 28809 | |
| <I/σ(I)> | 12.92 | 2.21 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 30.55% W/V PEG 6000, 0.1M TRIS-CL, PH 8.0 |
