5FF0

HydE from T. maritima in complex with S-adenosyl-L-cysteine and methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	[FeFe] hydrogenase maturase subunit HydE	polymer	358	41009.4	1	UniProt (Q9X0Z6) Pfam (PF04055)	Thermotoga maritima
2	B (A)	Fe4-Se4 cluster	non-polymer		539.2	1	Chemie (SFS)
3	C, D, E, F, K (A)	3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE	non-polymer		614.9	5	Chemie (CPS)
4	G, H (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	I (A)	METHIONINE	non-polymer		149.2	1	Chemie (MET)
6	J (A)	S-adenosyl-L-cysteine	non-polymer		370.4	1	Chemie (5X8)
7	L (A)	water	water		18.0	499	Chemie (HOH)

Sequence modifications

A: 2 - 348 (UniProt: Q9X0Z6)

PDB	External Database	Details
Met -9	-	initiating methionine
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ser 311	Cys 311	engineered mutation
Ser 319	Cys 319	engineered mutation
Ser 322	Cys 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	41009.4
Non-Polymers*	Number of molecules	10
	Total formula weight	4204.1
All*	Total formula weight	45213.5

*Water molecules are not included.