5FC3

Structural basis of cohesin cleavage by separase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	pAMK peptide	polymer	9	1065.2	1	BIRD (PRD_001260)	synthetic construct
2	B (B)	separase	polymer	619	68854.0	1	UniProt (G0SHM3) Pfam (PF03568)	Chaetomium thermophilum
3	C, D (A, B)	water	water		18.0	38	Chemie (HOH)

Sequence modifications

B: 1633 - 2223 (UniProt: G0SHM3)

PDB	External Database	Details
Met 1605	-	initiating methionine
Gly 1606	-	expression tag
Ser 1607	-	expression tag
Ser 1608	-	expression tag
His 1609	-	expression tag
His 1610	-	expression tag
His 1611	-	expression tag
His 1612	-	expression tag
His 1613	-	expression tag
His 1614	-	expression tag
Ser 1615	-	expression tag
Gln 1616	-	expression tag
Leu 1617	-	expression tag
Glu 1618	-	expression tag
Val 1619	-	expression tag
Leu 1620	-	expression tag
Phe 1621	-	expression tag
Gln 1622	-	expression tag
Gly 1623	-	expression tag
Pro 1624	-	expression tag
Leu 1625	-	expression tag
Gly 1626	-	expression tag
Ser 1627	-	expression tag
Gly 1628	-	expression tag
Arg 1629	-	expression tag
Pro 1630	-	expression tag
Lys 1631	-	expression tag
Lys 1632	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69919.2
All*	Total formula weight	69919.2

*Water molecules are not included.