5FC3
Structural basis of cohesin cleavage by separase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 149.148, 149.148, 115.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.820 - 3.100 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.128 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.099 |
| Number of reflections | 14250 |
| <I/σ(I)> | 14.6 |
| Completeness [%] | 99.9 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium citrate tribasic, PEG3350 |
