5FAW
T502A mutant of choline TMA-lyase
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Choline trimethylamine-lyase | polymer | 815 | 91556.1 | 2 | UniProt (Q30W70) Pfam (PF02901) Pfam (PF01228) In PDB | Desulfovibrio alaskensis | |
2 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
3 | A, B | MALONATE ION | non-polymer | 102.0 | 6 | Chemie (MLI) | |||
4 | A, B | CHOLINE ION | non-polymer | 104.2 | 2 | Chemie (CHT) | |||
5 | water | water | 18.0 | 2374 | Chemie (HOH) |
Sequence modifications
A, B: 53 - 846 (UniProt: Q30W70)
PDB | External Database | Details |
---|---|---|
Met 32 | - | initiating methionine |
Gly 33 | - | expression tag |
Ser 34 | - | expression tag |
Ser 35 | - | expression tag |
His 36 | - | expression tag |
His 37 | - | expression tag |
His 38 | - | expression tag |
His 39 | - | expression tag |
His 40 | - | expression tag |
His 41 | - | expression tag |
Ser 42 | - | expression tag |
Ser 43 | - | expression tag |
Gly 44 | - | expression tag |
Leu 45 | - | expression tag |
Val 46 | - | expression tag |
Pro 47 | - | expression tag |
Arg 48 | - | expression tag |
Gly 49 | - | expression tag |
Ser 50 | - | expression tag |
His 51 | - | expression tag |
Met 52 | - | expression tag |
Ala 502 | Thr 502 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 183112.2 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 866.6 | |
All* | Total formula weight | 183978.8 |