5FAW
T502A mutant of choline TMA-lyase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| A | 0033265 | molecular_function | choline binding |
| A | 0042426 | biological_process | choline catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0120525 | molecular_function | choline trimethylamine lyase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| B | 0033265 | molecular_function | choline binding |
| B | 0042426 | biological_process | choline catabolic process |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0120525 | molecular_function | choline trimethylamine lyase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 901 |
| Chain | Residue |
| A | SER746 |
| A | MET748 |
| A | HOH1231 |
| A | HOH1489 |
| A | HOH1646 |
| B | HOH1604 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 902 |
| Chain | Residue |
| A | HOH1061 |
| A | HOH1138 |
| A | HOH1175 |
| A | HOH1644 |
| A | ARG572 |
| A | LEU689 |
| A | HOH1018 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue MLI A 903 |
| Chain | Residue |
| A | ARG238 |
| A | THR633 |
| A | GLN636 |
| A | HOH1070 |
| A | HOH1356 |
| A | HOH1483 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MLI A 904 |
| Chain | Residue |
| A | LYS102 |
| A | ARG105 |
| A | TYR106 |
| A | HOH1239 |
| A | HOH1400 |
| A | HOH1596 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 905 |
| Chain | Residue |
| A | GLY49 |
| A | SER50 |
| A | HIS51 |
| A | HOH1044 |
| A | HOH1048 |
| A | HOH1075 |
| A | HOH1077 |
| A | HOH1107 |
| B | LYS71 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 906 |
| Chain | Residue |
| A | HIS519 |
| A | ASP535 |
| A | LEU536 |
| A | HOH1017 |
| A | HOH1179 |
| A | HOH1236 |
| A | HOH1525 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 907 |
| Chain | Residue |
| A | GLU242 |
| A | LEU244 |
| A | HOH1013 |
| A | HOH1260 |
| A | HOH1665 |
| A | HOH1786 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | binding site for residue CHT A 908 |
| Chain | Residue |
| A | TYR208 |
| A | ASP216 |
| A | GLY335 |
| A | MET487 |
| A | GLY488 |
| A | CYS489 |
| A | GLU491 |
| A | TYR506 |
| A | LEU698 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue MLI B 901 |
| Chain | Residue |
| B | SER50 |
| B | HIS51 |
| B | MET52 |
| B | GLY53 |
| B | HOH1072 |
| B | HOH1247 |
| site_id | AD1 |
| Number of Residues | 10 |
| Details | binding site for residue CHT B 902 |
| Chain | Residue |
| B | TYR208 |
| B | ASP216 |
| B | THR334 |
| B | GLY335 |
| B | GLY488 |
| B | CYS489 |
| B | GLU491 |
| B | ALA502 |
| B | TYR506 |
| B | LEU698 |
Functional Information from PROSITE/UniProt
| site_id | PS00850 |
| Number of Residues | 9 |
| Details | GLY_RADICAL_1 Glycine radical domain signature. VvRVAGYSA |
| Chain | Residue | Details |
| A | VAL816-ALA824 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1316 |
| Details | Domain: {"description":"PFL","evidences":[{"source":"PROSITE-ProRule","id":"PRU00887","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 242 |
| Details | Domain: {"description":"Glycine radical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00493","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Cysteine radical intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Glycine radical","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
